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Plasmodium berghei Vincke and Lips
Plasmodium berghei Vincke and Lips
規(guī)格:
貨期:
編號(hào):B210811
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Plasmodium berghei Vincke and Lips
商品貨號(hào) B210811
Strain Designations NK65D-CLBB13
Application
Vector borne research
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
derived from M. Yoeli strain NK65 by mosquito passage, Univ. Illinois, Urbana, pre-1978
Product Format frozen
Storage Conditions Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic Axenic
Type Strain no
Cryopreservation

Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.

  1. To harvest parasites, inject host with ketamine (0.1-0.2 ml).
  2. Open chest cavity to expose heart and exsanguinate via cardiac puncture using Yaeger's anticoagulant** (see below), 1 volume anticoagulant to 4 volumes blood.
  3. Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube.
  4. Aspirate supernatant using sterile Pasteur pipet.
  5. Resuspend pellet gently in remaining supernatant.
  6. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  7. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.).
  8. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Name of Depositor NE Alger
Year of Origin 1978
References

Branton MBStudies of clonal populations of NK65 Plasmodium berghei Ph.D. thesis, Univ. Illinois, 1978

梅經(jīng)理 17280875617 1438578920
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于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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