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Construct size (kb): 46.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt10
Intact vector size: 43.340
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.6 kb
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: lambda
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PCK1
Gene name: phosphoenolpyruvate carboxykinase 1
Contains complete coding sequence?: Y
Chromosome: 20; Localization: 20 q13.2-q13.31
Tissue: kidney
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.7
Cross references: DNA Seq. Acc.: L05144

Insert lengths(kb): 2.700000047683716

Tissue: kidney

Gene product: phosphoenolpyruvate carboxykinase 1, soluble(phosphoenolpyruvate carboxykinase (GTP)) [PCK1]

Restriction digests of the clone give the following sizes (kb): BamHI--16.0, 9.0, 6.8 (doublet), 5.6, 1.5; EcoRI--32.0, 11.0, 2.0, 0.8; HindIII--23.0, 9.3 (doublet), 4.5; NotI--34.0, 12.0;

PstI--11.0, 5.6 (doublet), 2.8 (doublet), 2.5 (doublet), 2.1, 1.8, 1.5, 1.3 (doublet, 1.2, 1.0 (doublet), 0.85; PvuII--11.0 (doublet), 4.5 (doublet), 4.1, 3.8, 1.8, 1.4; SacI--33.0, 13.0.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.15, 1.52; PvuII--0.13, 1.47; NotI--0.97; SacI--1.3; EcoRI--1.87.

Contains the complete coding sequence (nt 122-1990).

The 3' untranslated region contains a simple tandem repeat polymorphism that is highly polymorphic.