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pCMI37
pCMI37
規(guī)格:
貨期:
編號(hào):B209965
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pCMI37
商品貨號(hào) B209965
Designations pCMI37
Species Homo sapiens, human
Applications
RFLPs are observed under normal hybridization stringency.
Vector
Construct size (kb): 7.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 3.5
Gene product: DNA Segment, single copy [D7S368]
Alleles: A1, A2, C1, C2
Insert Size (kb) 3.5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--7.8; EcoRI--7.8; PvuII/HindIII--3.85, 3.35, 0.55; PvuII/PstI--3.45, 2.65, 1.35; ClaI--7.8; EcoRI/HindIII--3.9, 3.7; BamHI--uncut.
Maps near the centromere.
Polymorphisms are best resolved on 1.2% agarose gels with bromphenol blue run longer than 14 cm. RFLPs are observed under normal hybridization stringency. Washes should be done at 0.1X SSC, 0.1% SDS, 65C.
EcoRI, HindIII, PvuII, BglII, PstI and BamHI neither detect the site polymorphisms nor resolve the VNTR polymorphism adequately.
References

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pCMI37) on chromosome 7 [D7S368]. Nucleic Acids Res. 16: 4192, 1988. PubMed: 2897676

Yusuke Nakamura, personal communication

Peter J O'Connell, personal communication

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