国产成人综合久久久久久,亚洲国产成人久久综合碰,精品露脸国产偷人在视频,99久久精品免费看国产,国产免费一区二区三区在线观看,好吊妞国产欧美日韩免费观看,国产精品免费看久久久无码

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Heteromita obovata Lemmermann
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Heteromita obovata Lemmermann
Heteromita obovata Lemmermann
規(guī)格:
貨期:
編號:B209830
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Heteromita obovata Lemmermann
商品貨號 B209830
Strain Designations 2368
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
water directly above sediment at approx 2000 ft. in Hudson Canyon, Atlantic Ocean, 1992
Product Format test tube
Type Strain no
Comments
Protozoan indicators of sewage contamination
Medium ATCC® Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Temperature: 25.0°C
Cryopreservation
Cryoprotective Solution

DMSO ?????????????????????????????????????????????????????????????????????????????????? 2.0 ml

Fresh growth medium w/o bacteria???????????????????????????????? 8.0 ml

1.???? Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.

2. ?? Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min.

3.???? Adjust the concentration of cells at least 2 x 106/ml in fresh medium.

4.? ?? Mix the cell preparation and the cryoprotective solution in equal portions.

5.? ?? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6. ??? Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately -1°C/min.) ?

7.? ?? Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.

8. ??? To establish a culture from the frozen state place the vial in a 35°C water bath.? Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.?? Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 ml ATCC medium 802 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC? 700831) or Enterobacter aerogenes (ATCC? 13048).

9.     Incubate at 25°C with the cap screwed on tightly.

10.   Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 ml to 10.0 ml of bacterized ATCC medium 802.

11.   Follow the protocol for maintenance of culture.

Name of Depositor TK Sawyer
Year of Origin 1992
References

Sawyer TK, et al. Bacterial and protozoan indicators of sewage contamination of deepwater sediments at Hudson Canyon. J. Mar. Env. Eng. 2: 1-8, 1996.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
滁州市| 日土县| 武陟县| 盐山县| 浦江县| 铁力市| 临澧县| 连南| 松阳县| 黔西| 明星| 屯留县| 竹溪县| 富宁县| 大英县| 长宁区| 梓潼县| 盐亭县| 余江县| 垣曲县| 巴塘县| 儋州市| 嫩江县| 台江县| 师宗县| 确山县| 嘉峪关市| 株洲市| 云梦县| 汶上县| 盘锦市| 渝中区| 扶风县| 固原市| 家居| 皮山县| 临洮县| 集贤县| 交口县| 砚山县| 阿勒泰市|