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I
I
規(guī)格:
貨期:
編號(hào):B209753
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) I
商品貨號(hào) B209753
Designations I
Species Homo sapiens, human
Depositors JE Sadler
Vector
Construct size (kb): 7.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.900000095367432
Tissue: umbilical vein endothelium cell line
Gene product: von Willebrand factor(coagulation factor VIII VWF (von Willebrand factor)) [VWF]
Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2
Insert Size (kb) 2.900
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.2, 3.0; BamHI--7.2; EcoRI--7.2; PstI--4.2, 1.2, 0.75, 0.33; HindIII--7.2.
Probes I (ATCC 59782/59783), II (ATCC 59784/59785), III (ATCC 59786/59787) and IV (ATCC 59788/59789) constitute non-overlapping fragments of the human von Willebrand factor cDNA sequence that together span essentially the entire 9 kb cDNA.
Insert is a 2.9 kb EcoRI (linker)/BamHI fragment derived from lambdaHvWF6 containing about 210 bp 5' noncoding sequence and encoding a 22 residue signal peptide, 741 residues of vWAgII and the amino terminal 143 amino acids of the mature vWF subunit.
References

Shelton-Inloes BB, et al. Evolution of human von Willebrand Factor: cDNA sequence polymorphisms, repeated domains, and relationship to von Willebrand Antigen II. Biochem. Biophys. Res. Commun. 144: 657-665, 1987. PubMed: 3495266

Shelton-Inloes BB, et al. Gene deletions correlate with the development of alloantibodies in von Willebrand disease. J. Clin. Invest. 79: 1459-1465, 1987. PubMed: 3033024

J E Sadler, personal communication

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