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pSI4
pSI4
規(guī)格:
貨期:
編號(hào):B209724
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSI4
商品貨號(hào) B209724
Designations pSI4
Depositors JR Broach
Biosafety Level 1
Vector Information
Size (kb): 11.8999996185302700
Vector: pSI4 (plasmid)
Construction: pJDB219, pBR322
Marker(s):ampR,tetR,LEU2-d
Construct size (kb): 11.89999961853027
Features: marker(s): ampR, tetR, LEU2-d
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector for other uses
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--12.0; EcoRI--4.4, 4.1, 2.6, 0.8; HindIII--4.6, 3.4, 2.7, 1.3; PstI--12.0.
LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine).
High-copy YE-type shuttle vector.
Consists of the entire 2 micron circle genome cloned, at the EcoRI site of the small unique region, into the EcoRI site of pBR322. The leu2-d sequence (approximately 1.4 kb) is inserted into the PstI site of the 2 micron circle.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781

Broach JR. Construction of high copy yeast vectors using 2-micron circle sequences. Methods Enzymol. 101: 307-325, 1983. PubMed: 6310334

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
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