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pHRnX3.6
pHRnX3.6
規(guī)格:
貨期:
編號:B209700
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pHRnX3.6
商品貨號 B209700
Designations pHRnX3.6
Species Homo sapiens, human
Depositors JM Chirgwin
Vector
Construct size (kb): 6.300000190734863
Insert
DNA: genomic
Insert lengths(kb): 3.599999904632568
Gene product: renin [REN]
Alleles: H1, H2, J1, B1, B2, F1, F2, G1, K1, K2, G2, I1
Insert Size (kb) 3.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--3.6, 2.7; HindIII--5.9, 0.7; BamHI--2.8, 2.15, 1.3; PstI--4.0, 1.3, 1.2.
This insert includes exons 2 - 5.
Human renin cDNA cross-hybridizes with calf chymosin mRNA.
BglI, HindIII, BglII, TaqI, RsaI, and BstXI RFLPs are detected with a mix of the inserts from pHRnX0.8 (ATCC 57174), pHRnX3.6 (ATCC 57178), and pHRnES1.9 (ATCC 57176), apparently from base changes not within the coding regions of the gene.
Hybridization at reduced stringency results in cross-reaction with other members of the aspartyl protease gene family including cathepsin D (mapping to 11p15).
Enzyme(s) not detecting polymorphism: EcoRI, BanI, MlnI, BstEII, HphI, MspI, SstI, DraI, PvuII, MboII, ApaI, SinI, NciI, ScaI, XbaI, HinfI, NdeI, HincII. XmnI and PstI give uninterpretable results. Tested on a small sample.
References

Naftilan AJ, et al. A lack of genetic linkage of renin gene restriction fragment length polymorphisms with human hypertension. Hypertension 14: 614-618, 1989. PubMed: 2573574

Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294

Hobart PM, et al. Human renin gene: Structure and sequence analysis. Proc. Natl. Acad. Sci. USA 81: 5026-5030, 1984. PubMed: 6089171

McGill JR, et al. Chromosome localization of human renin gene (REN) by in situ hybridization. Cytogenet. Cell Genet. 45: 55-57, 1987. PubMed: 3297510

John M Chirgwin, personal communication

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