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<i>Clostridioides difficile</i> (Prevot) Lawson et al.

<i>Clostridioides difficile</i> (Prevot) Lawson et al.

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產(chǎn)品名稱(chēng)	Clostridioides difficile (Prevot) Lawson et al.
商品貨號(hào)	B243240
Deposited As	Clostridium difficile (Hall and O'Toole) Prevot
Strain Designations	W1194
Application	Enteric Research
Isolation	Feces of patient with pseudomembranous colitis, Belgium.
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Antigenic Properties	Serogroup A
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol®	no
Genotype	Toxinotype: 0
Type Strain	no
Comments	Presence of tcdA and tcdB genes confirmed by PCR. Binary toxin gene cdtB not amplified by PCR Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line ATCC CCL-81). Toxinotype 0 Ribotype 005
Toxins	Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line ATCC CCL-81).
Medium	ATCC^® Medium 2107: Modified Reinforced Clostridial
Growth Conditions	Temperature: 37°C Atmosphere: Anaerobic
Name of Depositor	M Delmee
References	Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688 Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

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