国产成人综合久久久久久,亚洲国产成人久久综合碰,精品露脸国产偷人在视频,99久久精品免费看国产,国产免费一区二区三区在线观看,好吊妞国产欧美日韩免费观看,国产精品免费看久久久无码

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > EBO-pSV2-neo
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
EBO-pSV2-neo
EBO-pSV2-neo
規(guī)格:
貨期:
編號(hào):B239996
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 EBO-pSV2-neo
商品貨號(hào) B239996
Designations EBO-pSV2-neo
Depositors RF Margolskee
Biosafety Level 1
Vector Information
Size (kb): 13.3599996566772500
Vector: EBO-pSV2-neo (plasmid)
Promoters: Promoter SV40 late
Construction: pSV2neo, p220-1 (EBV oriP, EBNA-1, hph)
Marker(s):G418R,ampR,hygR
Construct size (kb): 13.35999965667725
Features: marker(s): ampR, hygR, G418R
promoter: SV40 late
replicon: pMB1, EBV oriP
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0; SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6.
pcD cDNA libraries can be converted into EBO-pcD libraries by ligating the 7.4 kb SfiI fragment of EBO-pSV2-neo (containing oriP, EBNA-1, hph) into SfiI-linearized, phosphatased, pcD DNA.
A selectable, stably replicating shuttle vector for expression of cDNAs in eukaryotic cells. Maintained episomally.
The vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Margolskee RF, et al. Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells. Mol. Cell. Biol. 8: 2837-2847, 1988. PubMed: 2841588

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831

Okayama H, Berg P. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell. Biol. 3: 280-289, 1983. PubMed: 6300662

Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
慈溪市| 抚州市| 六盘水市| 黄平县| 寿阳县| 长阳| 修水县| 民勤县| 龙山县| 安宁市| 平陆县| 贵南县| 镇沅| 鹤壁市| 黄山市| 法库县| 苏尼特右旗| 新乡市| 龙陵县| 金阳县| 深泽县| 漾濞| 乐清市| 南投县| 泸西县| 呼玛县| 上高县| 福贡县| 沙坪坝区| 山阳县| 施秉县| 莆田市| 平罗县| 绍兴县| 鹤峰县| 浦县| 昔阳县| 灵山县| 长武县| 道孚县| 兴文县|