pSP65:HSV #11 [partial length clone of hop stunt viroid]
商品貨號(hào)
B228074
Designations
pSP65:HSV #11 [partial length clone of hop stunt viroid]
Species
Hop stunt viroid
Depositors
RA Owens, Beltsville Agricultural Research Center
Applications
To use as a template, the plasmid must be linearized with either PstI or HindIII.
Vector
Name of construct: pSP65:HSV #11
Excise insert: BamHI+PstI
Size of construct (kb): 3.26
Markers: ampR
Name of vector: pSP65
Intact vector size: 3.010
Type of vector: plasmid
Vector end: BamHI
Vector end: PstI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range:Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: SP6
replicon: pMB1
terminator: none
Insert
Genome: hop stunt viroid
Gene name: genome, partial
Contains complete coding sequence?: U
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.26
Insert Size (kb)
0.260
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains internal SstII and XhoI sites.
Restriction digests of the clone give the following sizes (kb): BamHI/PstI--3.0, 0.29; PstI--3.2; HindIII--3.2.
To use as a template, the plasmid must be linearized with either PstI or HindIII.
A partial length (about 260 bp) fragment of Hop Stunt Viroid cDNA was cloned into BamHI/PstI of pSP65; noninfectious. Constructed by R.W. Hammond.
E. Shikata provided the original Hop Stunt Viroid cDNA.
Classification
Hostuviroid
References
Ohno T, et al. Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy. Nucleic Acids Res. 11: 6185-6197, 1983. PubMed: 6312412
