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pTFE3-1.9
pTFE3-1.9
規(guī)格:
貨期:
編號:B222328
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pTFE3-1.9
商品貨號 B222328
Designations pTFE3-1.9
Species Homo sapiens, human
Vector
Construct size (kb): 4.78000020980835
Insert
DNA: cDNA
Insert lengths(kb): 1.815000057220459
Tissue: B lymphocyte
Gene product: transcription factor binding to IGHM enhancer 3(transcription factor for IgH enhancer) [TFE3]
Alleles: A1, A2
Insert Size (kb) 1.815
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
The insert includes the following restriction sites (position according to the published sequence): PstI--677, 1859; NcoI--687, 2447; StuI--1111; HindIII--2216.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 1.8; HindIII--4.5, 0.32; SmaI--4.8; PstI--3.8, 1.2; XhoI--4.8.
The insert extends from a site 22 base pairs 5' to the PstI site at nucleotide 677 of the published sequence through the 3' end. It encodes amino acids 220 through 536 and includes the 3' untranslated region.
Enzyme(s) not detecting polymorphism: BglI, BglII, DraI, EcoRI, MspI, PstI (20 unrelated X chromosomes), and TaqI (10 unrelated X chromosomes).
References

Beckmann H, et al. TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif. Genes Dev. 4: 167-179, 1990. PubMed: 2338243

Puck JM, et al. A high-frequency RFLP at the human TFE3 locus on the X chromosome. Nucleic Acids Res. 19: 684, 1991. PubMed: 1672758

Paula S Henthorn, personal communication

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