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P-1 [P1]
P-1 [P1]
規(guī)格:
貨期:
編號(hào):B218678
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 P-1 [P1]
商品貨號(hào) B218678
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain BALB/c
Applications
The antibody reacts with the small subunit of bovine mu-caplain and m-calpain.
It cross-reacts with the small subunit of human and canine mu-caplain and m-calpain.
The antibody can be used for immunochemical and Western blot applications.
It is a useful tool for detecting and localizing the small subunit of calpain.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified bovine myocardial calpain 2
Spleen cells were fused with FOX-NY myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the small subunit of calpain
Cellular Products
immunoglobulin; monoclonal antibody; against the small subunit of calpain
Comments
Animals were immunized with purified bovine myocardial calpain 2
Spleen cells were fused with FOX-NY myeloma cells.
The antibody reacts with the small subunit of bovine mu-caplain and m-calpain.
It cross-reacts with the small subunit of human and canine mu-caplain and m-calpain.
The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the small subunit of calpain.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 x 104 viable cells/mL. Maintain cell concentration between 3 x 104 and 3 x 105 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1 kappa , IgG1; kappa light chain
Population Doubling Time 24 hours
Name of Depositor R Lane, RL Mellgren
Deposited As mouse (B cell); mouse (myeloma)
Year of Origin 1986
References

Mellgren RL, Lane RD. Myocardial calpain 2 is inhibited by monoclonal antibodies specific for the small, noncatalytic subunit. Biochim. Biophys. Acta 954: 154-160, 1988. PubMed: 2835105

Zhang W, Mellgren RL. Calpain Subunits Remain Associated during Catalysis. Biochem. Biophys. Res. Commun. 227: 890-896, 1996. PubMed: 8954990

Zhang W, et al. The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J. Biol. Chem. 271: 18825-18830, 1996. PubMed: 8702541

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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胡經(jīng)理 13345964880 2438244627
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于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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