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Enterovirus D68
Enterovirus D68
規(guī)格:
貨期:
編號:B217707
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Enterovirus D68
商品貨號 B217707
Classification Picornaviridae, Enterovirus, Enterovirus D
Agent Enterovirus D68
Strain US/KY/14-18953
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1825 images
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
RD cells (ATCC® CCL-136™)
Growth Conditions Temperature: 33°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01-0.001). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 2-3 days
Name of Depositor Centers for Disease Control and Prevention
Source Nasopharyngeal swab from patient with respiratory illness, Kentucky, September 2014
References

Brown BA, et al. Seven Strains of Enterovirus D68 Detected in the United States during the 2014 Severe Respiratory Disease Outbreak. Genome Announc 2(6): e01201-14, 2014. PubMed: 25414503

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