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HFBDV22
HFBDV22
規(guī)格:
貨期:
編號:B216862
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HFBDV22
商品貨號 B216862
Designations HFBDV22
GenBank Number

T06562

Species Homo sapiens, human
Vector
Construct size (kb): 4.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TLE3*
Gene name: transducin-like enhancer protein (enhancer of split)
Contains complete coding sequence?: N
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M99438; EST: EST04451
Nucleotides 1-335 of the insert correspond to
nucleotides 2007-2341 of M99438 with 97% identity.
DNA Seq. Acc.: T06562; EST: EST04451; Primer: M13-21; THC
id: THC117710
DNA Seq. Acc.: T06563; EST: EST04452; Primer: M13 Reverse
Insert lengths(kb): 0.800000011920929
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: transducin-like enhancer protein (enhancer of split)( transducin-like enhancer of split 3, transducin-like enhancer 3) [TLE3*]
Insert Size (kb) 0.800
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.0; EcoRI--3.2, 0.8.
Verified to contain EST04451 by single-pass sequencing.
Based on the size of the insert and the alignment with GenBank accession M99438 (2357 nt, CDS 22-2340), this clone appears to contain part of the coding region and the 3' untranslated region. EST04452 does not match a nucleotide sequence record.
References

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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