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L5178-S (LY-S)
L5178-S (LY-S)
規(guī)格:
貨期:
編號(hào):B164947
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 L5178-S (LY-S)
商品貨號(hào) B164947
Organism Mus musculus, mouse
Tissue thymus
Cell Type chemically induced
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain DBA/2
Storage Conditions liquid nitrogen vapor phase
Derivation This line was derived from the X-ray resistant L5178-R line (ATCC CRL-1722).

The parental cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse.
Tumorigenic Yes
Effects
Yes, in DBA/2 mice (but only weakly).
Comments The cells are sensitive to X-rays, ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD).
Complete Growth Medium Fischer's medium with 0.1 g/L sodium pyruvate and 1.125 sodium bicarbonate, 90%; horse serum, 10%
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 104 cells/mL and maintain between 5 x 103 and 7 x 105 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium, 90%; DMSO, 10%
Name of Depositor JZ Beer
Deposited As Mus musculus
References

Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899

Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332

Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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