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DII 33.1
DII 33.1
規(guī)格:
貨期:
編號(hào):B164359
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 DII 33.1
商品貨號(hào) B164359
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with carbohydrate moieties on the human insulin receptor.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human placental insulin receptor
Cellular Products
immunoglobulin; monoclonal antibody; against human placental insulin receptor
Tumorigenic Yes
Effects
Yes, in (BALB/c X SJL/J)F1 mice
Comments
Animals were immunized with Wheat Germ Agglutinin bound fraction of a placental membrane extract.
Spleen cells were fused with NS0 myeloma cells.
The antibody reacts with carbohydrate moieties on the human insulin receptor.
The antibody may cross-react with other cell surface glycoproteins.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing

    Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 to 1 x 105 viable cells/ mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.

    Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
    Cryopreservation

    Complete growth medium described above supplemented with 10% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
    Culture Conditions
    Temperature: 37°C
    Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
    Isotype IgG1
    Name of Depositor S Ganguly
    Deposited As mouse (B cell); mouse (myeloma)
    References

    Ganguly S, et al. Insulin-dependent tyrosine protein kinase: cellular distribution and copurification with the insulin receptor. Curr. Top. Cell. Regul. 27: 83-94, 1985. PubMed: 3937668

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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