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55-6
55-6
規(guī)格:
貨期:
編號:B163785
品牌:Mingzhoubio

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產(chǎn)品名稱 55-6
商品貨號 B163785
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
Suzanne Epstein that reacts with the gp120 of HIV-1.
In ELISA the antibody reacts strongly with rgp120 IIIB and weakly to gp120 MN.
It binds to the C1 region (positions 105 - 117) gp120 synthetic peptide used to map antibody reactivity and was not able to cross block any other antibodies in the panel.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
55-6 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1.
Genes Expressed
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120
Cellular Products
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were sequentially immunized with recombinant gp120 (rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB, SF2, and Z6).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
55-6 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1.
In ELISA the antibody reacts strongly with rgp120 IIIB and weakly to gp120 MN.
It binds to the C1 region (positions 105 - 117) gp120 synthetic peptide used to map antibody reactivity and was not able to cross block any other antibodies in the panel.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
    Medium Renewal: Two to three times weekly.
    Cryopreservation

    Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
    Culture Conditions
    Temperature: 37°C
    Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
    Isotype IgG2a
    Name of Depositor SL Epstein
    Deposited As mouse (B cell); mouse (myeloma)
    References

    Reeves JP, et al. Mouse monoclonal antibodies to human immunodeficiency virus glycoprotein 120 generated by repeated immunization with glycoprotein 120 from a single isolate, or by sequential immunization with glycoprotein 120 from three isolates. Hybridoma 14: 235-242, 1995. PubMed: 7590785

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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