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4B2
4B2
規(guī)格:
貨期:
編號(hào):B163759
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
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產(chǎn)品名稱 4B2
商品貨號(hào) B163759
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications

The antibody reacts with all types of human leukocytes, but not with non-leukocytes.

The antibody binds to protein A.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with human T cells.

Spleen cells were fused with P3U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)
Cellular Products
immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)
Tumorigenic Yes, in irradiated pristane primed (BALB/c X DBA/2)F1 mice.
Comments

The antibody reacts with all types of human leukocytes, but not with non-leukocytes.

The antibody binds to protein A.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Culture Conditions Temperature: 37°C
Isotype IgG2a
Name of Depositor RM Steinman
Deposited As mouse (B cell); mouse (myeloma)
References

. Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ. Press; 1987.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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