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14E5
14E5
規(guī)格:
貨期:
編號(hào):B163578
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 14E5
商品貨號(hào) B163578
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with human vascular endothelial cells, mature peripheral blood macrophages and DHL-2 cells, but does not react with bovine endothelial cells or human B or T lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human endothelium and macrophage
Cellular Products
immunoglobulin; monoclonal antibody; against human endothelium and macrophage
Comments
Animals were immunized with human umbilical vein endothelial cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with human vascular endothelial cells, mature peripheral blood macrophages and DHL-2 cells, but does not react with bovine endothelial cells or human B or T lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: RPMI medium 1640, 85%; fetal bovine serum, 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor YA Reid
Deposited As mouse (B cell); mouse (myeloma)
References

Hamburger AW, et al. Isolation and characterization of monoclonal antibodies reactive with endothelial cells. Tissue Cell 17: 451-459, 1985. PubMed: 3901396

梅經(jīng)理 17280875617 1438578920
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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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