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pCMV4
pCMV4
規(guī)格:
貨期:
編號(hào):B162456
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCMV4
商品貨號(hào) B162456
Designations pCMV4
Depositors DL Davis
Biosafety Level 1
Vector Information
Size (kb): 4.8740000724792480
Vector: pCMV4 (phagemid)
Promoters: Promoter CMV
Construction: pTZ18R, bacteriophage f1, CMV promoter
Marker(s):ampR
Construct size (kb): 4.874000072479248
Features: marker(s): ampR
promoter: CMV
replicon: f1
MCS: BglI...SmaI
transcription terminator: hGH
enhancer: SV40
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 1.3; BglII--4.9; BglII/SmaI--4.9.
Should be grown in media containing ampicillin at a final concentration of 1 mg/ml to improve plasmid yields.
May be used in essentially all cultured cells in transient transfection assays, to establish permanent cell lines and to construct transgenic mice.
This vector contains a translational enhancer in the sequence prior to the polylinker.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Andersson S, et al. Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778

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梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
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